L-Tryptophan Improves Germination and Early Growth of Glycine max Seedlings Subjected to Cold Test

DOI: 10.22161/ijeab.105.9

Abstract:
The genetic potential of seeds does not inherently ensure uniform establishment and optimal stand quality in commercial crop fields. This challenge has led to the development of various agricultural inputs, often applied directly to seeds, including L-tryptophan. The present study was conducted using a completely randomized factorial experimental design with two factors and four replications. The first factor comprised two levels: the presence (CT) and absence (ACT) of a cold test. The second factor included four concentrations of L-tryptophan: 0, 0.02, 0.04, and 0.08 g kg-1 of seeds. Soybean seeds were sown on Germitest® paper substrate and maintained in a germination chamber. Evaluations included germination rate (normal and abnormal seedlings, as well as hard or dead seeds), primary root length, shoot length and dry matter. The application of L-tryptophan exhibited a protective effect on soybean seeds subjected to cold stress, indicating its potential as an effective seed treatment for crops expected to encounter low temperatures and high soil moisture during germination.

Keywords:
amino acid, cold test, germination test, soybean, seed vigor.

Article Info:
Received: 12 Aug 2025; Received in revised form: 10 Sep 2025; Accepted: 15 Sep 2025; Available online: 19 Sep 2025

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